293T Cell Lysate

- Overview
  - 293T cells are a human embryonic kidney (HEK) cell line that expresses the large T antigen. The large T antigen is commonly used for transfection assays and is required for transient transfection of plasmids with an SV40 origin of replication. 293T cells are able to grow well in reduced serum medium or in medium lacking calcium ions. In addition, 293T cells tend to grow at a faster rate and to a higher cell density than the parent 293 cell line, resulting in increased amounts of endogenously expressed protein.
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Cell Lysates
    
    Formulation
    
    Liquid. In SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
    
    Concentration
    
    2mg/ml (determined by BCA assay)
    
    Other Properties
    
    Quality Control: Western Blot analyzed by both GAPDH and Beta tubulin antibody
    
    Storage
    
    Aliquot the lysate into single tube and then storing them -80°C or below. Avoid repeated freezing and thawing cycles.
    
    * For research use only. Not intended for human, diagnostics or veterinary use.
- Applications
  - Application Description
    
    Heat lysate to 95°C for 5 minutes and rapidly cooling before use.