Guanidine Thiocyanate, Ultrapure

- Overview
  - Guanidine Thiocyanate, Ultrapure is a freely soluble chaotropic agent routinely used for DNA/RNA extraction protocols.
    
    This product is considered a dangerous good. Quantities above 1 gram may be subject to additional shipping fees. Please contact Customer Service for details.
    
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    Background
    
    Extraction of cellular DNA and RNA releases DNases and RNases. Guanidine Thiocyanate is used during cell lysis because of its ability to irreversibly inactivate degrading nucleases. In addition to its chaotropic properties, Guanidine Thiocyanate has also shown to inactivate enveloped viruses and some non-enveloped viruses.
- Properties
  - CAS Number
    
    593-84-0
    
    Molecular Formula
    
    CH5N3 • HSCN
    
    Molecular Weight
    
    118.16
    
    Appearance
    
    White crystalline powder
    
    Solubility
    
    Water: Freely soluble
    
    Other Properties
    
    Source: Synthetic
    PH: (6M): 4.5-6.0
    Loss on Drying: ≤0.3%
    Absorbance: (6M) 280nm : ≤0.5
    Melting Point: 115-122 °C
    Purity Level: ≥99.0%
    
    Storage
    
    2-8 °C
    
    * For research use only
- Applications
  - Application Description
    
    Microbiology Applications: Guanidine Thiocyanate is routinely used for DNA/RNA extraction protocols to prevent damage by degredative enzymes.
    
    Eukaryotic Cell Culture Applications: Guanidine Thiocyanate can be used to isolate RNA from both cell culture and tissue form human and animal sources. Authors extracted a protocol for extracting RNA from human placental tissue and from primary and transformed cell lines of human and animal origin, without any ultracentrifugation steps or phenol extractions. Human skin fibroblasts, primary hepatocytes from rats, and Fu5AH rat hepatoma cell line were used and the RNA extracted was intact, with good yield, iRNAse free and not contaminated by DNA. It was suitable for RT-PCR, Northern blotting, and in vitro protein translation (Zolfaghari et al, 1993).

MSDS

Documents

MSDS-EU

Guanidine Thiocyanate, Ultrapure

Guanidine Thiocyanate, Ultrapure

Background

Documents

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