GST-NEMO (UBAN)

- Overview
  - GST was fused on the N-terminus of the linear polyubiquitin chain binding domain of NEMO en-compassing amino acids 183-339. This fusion protein can be used for in vitro GST pulldown as-says and for enrichment of cellular proteins conjugated with linear polyubiquitin chains in whole cell or tissue lysates. GST-NEMO (UBAN) can be precipitated using glutathione resin. After washing, GST-NEMO (UBAN)and its bound proteins can be eluted by a buffer containing 10 mM glutathione.
    
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- Properties
  - Protein Name
    
    IKBKG; IP; IP1; IP2; FIP3; IPD2; NEMO; FIP-3; Fip3p; AMCBX1; ZC2HC9; IKK-gamma
    
    Source
    
    Bacterial recombinant
    
    Formulation
    
    20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
    
    Storage
    
    Store at -80°C; avoid multiple freeze-thaw cycles
    
    Concentration
    
    See tube label
    
    Molecular Weight
    
    18.4 kDa
    
    Sequence
    
    NCBI RefSeq: NM_001099857
    
    * Research Use Only