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Overview
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6xHis was fused on the N-terminus of the linear polyubiquitin chain binding domain of NEMO encompassing amino acids 183-339. This fusion protein can be used for in vitro pulldown assays and for enrichment of cellular proteins conjugated with linear polyubiquitin chains in whole cell or tissue lysates. 6xHis-NEMO (UBAN) can be precipitated using Nickel resin. After washing, 6xHis-NEMO (UBAN) and its bound proteins can be eluted by a buffer containing 200 mM imidazole.
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Overview
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