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Overview
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Reactive oxygen species (ROS) are produced as an inevitable byproduct of oxidative phosphorylation in healthy and cancer cells. Cancer has higher ROS from increased metabolic activity, mitochondrial dysfunction, increased cellular receptor signaling and other oncogene activity, but cancer can tolerate it by increase the expression of ROS scavenger. Here we generated genetically encoded H2O2 sensor that has high sensitivity for ROS, allows for the ratiometric real-time analysis of ROS generation in living cells. roGFPs sensor can be excited by blue light with wavelength 405nm and 488nm and detected at 510nm. The ratio of 405nm/488nm indicates the redox- status within the cells.
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Overview
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