Genetically Encoded Calcium Indicator - ER/SR

Sub-cellular compartments such as the mitochondria, the endoplasmic reticulum (ER), and the SR, have calcium ion (Ca2+) concentrations ranges spanning from low micromolar to high millimolar. In compartments with high Ca2+ concentrations, fluorescent indicators which are optimized for the detection of cytoplasmic Ca2+ (typically in the 0.1 to 10 μM range) become saturated and unresponsive to physiologically relevant changes in Ca2+ concentration. To address this problem, substantial research effort has gone into developing low affinity Ca2+ indicators, including genetically encoded fluorescent proteins (FP). In contrast to synthetic dye-based indicators, FP-based indicators are delivered to the cell as their corresponding DNA coding sequences and can include additional sequences for expression in specific tissues or targeted to specific subcellular compartments.

Please contact us at for specific academic pricing.