G-361 Cell Lysate

G-361 Cell Lysate

Catalog Number:
CL01443514NKM
Mfr. No.:
ATGL0012
Price:
$260
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      • Overview
        • G-361 cells are established from a malignant melanoma of a 31 year-old male. The cells produce melanin for up to 50 population doublings. The Y chromosome could not be detected in this cell line by short tandem repeat (STR) -PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

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      • Properties
        • Categories
          Cell Lysates
          Formulation
          Liquid. In SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
          Concentration
          2mg/ml (determined by BCA assay)
          Other Properties
          Quality Control: Western Blot analyzed by both GAPDH and Beta tubulin antibody
          Storage
          Aliquot the lysate into single tube and then storing them -80°C or below. Avoid repeated freezing and thawing cycles.

          * For research use only. Not intended for human, diagnostics or veterinary use.

      • Applications
        • Application Description
          Heat lysate to 95°C for 5 minutes and rapidly cooling before use.

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