Fructan Assay Kit

- Overview
  - The Fructan Assay Kit is suitable for the specific measurement of fructan in plant extracts, animal feed and food products containing starch, sucrose and other sugars. It is used in three validated methods for the determination of fructan: AOAC method 999.03 (foods), AOAC method 2018.07 (Animal Feed) and AOAC method 2016.14 (infant formula and adult nutritionals).
    
    New, improved procedure.
    
    In the most recent development, a recombinant endo-levanase has been incorporated into the fructanase mixture, extending the use of the method to the measurement of levan-type fructans as are present in grasses such as timothy, cocksfoot, ryegrass and red fescue.
    
    The method described in this booklet employs ultra-pure, recombinant enzymes and specifically measures fructans including inulin-type fructans from chicory, dahlia, jerusalem artichoke; highly branched fructans from onion and wheat stems and leaves; and levan-type fructans from pasture grasses such as timothy grass. The enzymes employed are completely devoid of contaminating enzymes active on β-glucan or gluco-oligosaccharides.
    
    Please contact us at for specific academic pricing.
- Properties
  - Details
    
    Limit of Detection: 0.16g/100g
    
    Storage
    
    Short term stability: 2-8°C
    Long term stability: See individual component labels
    
    Stability
    
    > 2 years under recommended storage conditions
    
    * For research use only.
- Applications
  - Application Description
    
    Flours, infant formula, animal feed, pet foods, plant materials (e.g. onion), food products and other materials.
- Reference
  - 1.Determination of Fructan (Inulin, FOS, Levan, and Branched Fructan) in Animal Food (Animal Feed, Pet Food, and Ingredients): Single-Laboratory Validation, First Action 2018.07. McCleary, B. V., Charmier, L. M. J., McKie, V. A., Ciara McLoughlin, C. & Rogowski, A. (2019). Journal of AOAC International, 102(3), 2019 883.
    2.Measurement of carbohydrates in grain, feed and food. McCleary, B. V., Charnock, S. J., Rossiter, P. C., O’Shea, M. F., Power, A. M. & Lloyd, R. M. (2006). Journal of the Science of Food and Agriculture, 86(11), 1648-1661.
    3.Measurement of total fructan in foods by enzymatic/spectrophotometric method: Collaborative study. McCleary, B. V., Murphy, A. & Mugford, D. C. (2000). Journal of AOAC International, 83(2), 356-364.
    4.Measurement of total starch in cereal products by amyloglucosidase-alpha-amylase method: collaborative study. McCleary, B. V., Gibson, T. S. & Mugford, D. C. (1997). Journal of AOAC International, 80, 571-579.
    5.Identification of inulin-responsive bacteria in the gut microbiota via multi-modal activity-based sorting. Riva, A., Rasoulimehrabani, H., Cruz-Rubio, J. M., Schnorr, S. L., von Baeckmann, C., Inan, D., et al. (2023). Nature Communications, 14(1), 8210.
    6.Agave amica a potential model for the study of agavins metabolism. Salomé-Abarca, L. F., Márquez-López, R. E. & López, M. G. (2023). Scientific Reports, 13(1), 19888.
    7.Trash or treasure: Rhizome conservation during drought. Lubbe, F. C., Bitomský, M., Bartoš, M., Marešová, I., Martínková, J. & Klimešová, J. (2023). Functional Ecology, In Press
    8.High fructan barley lines produced by selective breeding may alter β-glucan and amylopectin molecular structure. Jayarathna, S., Jin, Y., Dotsenko, G., Fei, M., Andersson, M., Andersson, A. A., Sun, C. & Andersson, R. (2023). Carbohydrate Polymers, 316, 121030.
    9.Electrosprayed microparticles from inulin and poly (vinyl) alcohol for colon targeted delivery of prebiotics. Saud, K. T., Xu, J., Wilkanowicz, S., He, Y., Moon, J. J. & Solomon, M. J. (2023). Food Hydrocolloids, 108625.
    10.Reduction of FODMAPs and Amylase-Trypsin Inhibitors in Wheat: A review. Boakye, P. G., Okyere, A. Y. & Annor, G. A. (2023). Food Hydrocolloids for Health, 3, 100117.
    11.Fecal microbiota composition affects in vitro fermentation of rye, oat, and wheat bread. Pirkola, L., Dicksved, J., Loponen, J., Marklinder, I. & Andersson, R. (2023). Scientific Reports, 13(1), 99.
    12.Composition of Polysaccharides in Hull-Less Barley Sourdough Bread and Their Impact on Physical Properties of Bread. Reidzane, S., Gramatina, I., Galoburda, R., Komasilovs, V., Zacepins, A., Bljahhina, A., Kince T., Traksmaa, A. & Klava, D. (2022). Foods, 12(1), 155.
    13.Asparagus Fructans as Emerging Prebiotics. Hamdi, A., Viera-Alcaide, I., Guillén-Bejarano, R., Rodríguez-Arcos, R., Muñoz, M. J., Monje Moreno, J. M. & Jiménez-Araujo, A. (2023). Foods, 12(1), 81.
    14.EPS-Producing Microorganisms from Louisiana’s Crusher Juice and the Effect of Processing Conditions on EPS Production. Aita, G. M. & Moon, Y. H. (2022). Sugar Tech, 1-9.
    15.Self-assembly levan, as a natural active bio-nanocarrier. Lewińska, A., Domżał-Kędzia, M. & Łukaszewicz, M. (2023). Sustainable Chemistry and Pharmacy, 31, 100936.

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Fructan Assay Kit

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