FLEXIQuant PLUS Expression Kit

FLEXIQuant PLUS Expression Kit

Catalog Number:
CES1237501CEL
Mfr. No.:
CFS-FLEX-MS
Price:
$1,680
  • Size:
    1 set
    Quantity:
    Add to Cart:
      • Overview
        • This kit was designed to be user-friendly even for first-time users.
          Our wheat germ cell-free expression system allows you to easily make full-length isotope-labeled MS standards.
          Features:
          • Incorporates stable 13C and 15N isotopes-labeled lysine and arginine residues suitable for working with trypsin digestion in bottom-up proteomics
          • Very high labeling efficiency with incorporation rates of over 99%
          • No need to purchase 13C and 15N stable isotope-labeled amino acids as they are already added to translation system
          • Special expression and control vector provided to utilize FLEX-tag for absolute protein quantification by FLEXIQuant method
          • All reagents included to directly prepare labeled proteins from DNA template
          • Premixed reagents to synthesize proteins with just a few pipetting steps
          • Translation buffer provided in special reaction cups for bilayer reactions
          • Target protein expression can be confirmed through SDS-PAGE/CBB staining
          • Kit contains reagents for 8 translation reactions on 226 µl reaction scale
          Recommended for:
          • Users who require full-length isotope-labeled MS standards easily using wheat germ cell-free expression system

          All reagents are already aliquoted to provide the amounts needed per reaction.
          This makes reaction setup easy and avoids unnecessary freeze-thawing of sensitive reagents.
          Simply cut off the number of tubes needed for each experiment.
          General flow of protein expression using FLEXIQuant PLUS Expression Kit
          *1 : WEPRO9240 for MS is based on the WEPRO8240 with the addition of 13C and 15N-labeled lysine, arginine, and Creatine Kinase.
          *2 : SUB-AMIX SGC for MS includes 18 types of non-labeled amino acids excluding lysine and arginine.
          *3 : Transcription Premix LM is based on Transcription Buffer LM, with the addition of NTP Mix, SP6 RNA Polymerase and RNase Inhibitor.

          Please contact us at for specific academic pricing.

          More Details

      • Properties
        • Categories
          Protein labeling for protein mass spectorometry (MS)
          Storage
          -80 °C

          * The product is for research purpose only; not for use in diagnostic testing and use in human.

      • Applications
        • Application
          Used to make full-length isotope-labeled MS Standards

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