FITC-labeled Fibrinogen

- Overview
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    Background
    
    Fibrinogen (factor I) is a plasma glycoprotein that is converted by thrombin into fibrin by cleaving fibrinopeptides A+B during blood coagulation. Fibrin forms the blood clot and is subsequently cross linked by factor XIIIa. Human fibrinogen is a hexamer composed of three pairs of chains (α2β2γ2) with a molecular weight of 340 kDa.
- Properties
  - Source
    
    Human
    
    Storage
    
    Storage for several months is possible at ≤ - 20°C. Delivery is possible at ambient temperature
    
    Note
    
    INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.
    
    Class
    
    Protein Substrates
    
    Reconstitution
    
    Add at least the volume of H2O the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots.
    
    Activity
    
    Ca2+, Thrombin
- Applications
  - Application Description
    
    The FITC-labelled fibrinogen may be used as substrate for FXIII in a crosslinking reaction. The crosslinking of fibrin followed by fibrinolysis gives information about clot-rigidity and therefore about the degree of clot-formation catalysed by FXIII.
- Reference
  - Mutch et al., J Thromb Haemost. 2010, 8:2017-24