Fast Glucose Oxidase Activity Assay (Colorimetric/Fluorometric)

Glucose oxidase (GOD) catalyzes the oxidation of glucose from D-glucose to D-glucono-δ-lactone. Physiologically, it aids in the breakdown of glucose into smaller metabolites. It is widely used in electrochemical glucose sensors designed for diabetes patients. Simple, direct, and high-throughput assays for measuring glucose oxidase activity find wide applications in research and drug discovery.
Our Fast Glucose Oxidase Activity Assay uses a single working reagent that combines the glucose oxidase reaction and color reaction in one step. The change in color intensity of the reaction product at 570nm or fluorescence intensity at λ ex/em = 530/585 nm is directly proportional to glucose oxidase activity in the sample. The fluorometric assay is more sensitive than the colorimetric assay.

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