Name: EZ Cap™ Reagent AG
Price: 273.00 USD

- Overview
  - Please contact us at for specific academic pricing.
    
    Background
    
    EZ Cap Reagent AG is a co-transcription capping reagent for in vitro transcription of 5'capped mRNA to produce Cap 1 structure. Cap AG has been shown in the literature to provide a capping efficiency of up to 98%.
    EZ Cap AG requires an AG initiator. EZ Cap AG typically provides >90% capped material generating a naturally occurring Cap 1 structure.
    EZ Cap is compatible with wild-type T7 polymerase. 5 mM EZ Cap and rNTPs is recommended.
    Template design is an integral part of any transcription. EZ Cap reagent AG can be used with a specific initiating sequence 5’ AG 3’.
    Final capping efficiency depends on the EZ Cap Reagent, DNA template and final mRNA sequence. Secondary structure due to RNA length and base composition can affect final capping efficiency.
- Properties
  - Categories
    
    Used for co-transcriptional capping of mRNA to form Cap 1 structure, m7G(5')ppp(5')(2'OMeA)pG.
    
    Alternative Name
    
    EZ Cap™ Reagent AG for co-transcriptional capping of mRNA,m7G(5')ppp(5')(2'OMeA)pG
    
    Molecular Formula
    
    C32H43N15O24P4 (free acid)
    
    Molecular Weight
    
    1145.6 (free acid)
    
    Appearance
    
    Solution
    
    Purity
    
    Purity ≥ 95% by AX-HPLC
    
    Storage
    
    Store at -20°C or below
    
    SMILES
    
    O=C1NC(N)=NC2=C1N=CN2C3C(O)C(O)C(COP([O-])(OC4C(OC)C(N5C(N=CN=C6N)=C6N=C5)OC4COP([O-])(OP([O-])(OP([O-])(OCC7OC(N8C=[N+](C)C9=C8N=C(N)NC9=O)C(O)C7O)=O)=O)=O)=O)O3
    
    * For Research Use Only
- Reference
  - 1. Yu Zhang, Xiaodong Xi, et al. "Chemically modified in-vitro-transcribed mRNA encoding thrombopoietin stimulates thrombopoiesis in mice." Molecular Therapy: Nucleic Acids. PMID: 36090760
    2. Shudan Tan, Xue Hu, et al. "Preclinical evaluation of RQ3013, a broad-spectrum mRNA vaccine against SARS-CoV-2 variants." biorxiv.
    3. Jing Lu, Guoliang Lu, et al. "A COVID-19 mRNA vaccine encoding SARS-CoV-2 virus-like particles induces a strong antiviral-like immune response in mice." Cell Res. 2020;1-4. PMID: 32801356

Documents

COA (Certificate Of Analysis)

MSDS (Material Safety Data Sheet)

EZ Cap™ Reagent AG

EZ Cap™ Reagent AG

Background

Documents

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