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EZ Cap™ Reagent AG (3' OMe)

EZ Cap™ Reagent AG (3' OMe)

Catalog Number:
NNNA373595APE
Mfr. No.:
APE-B8178
Price:
$330
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      • Overview

        • Please contact us at for specific academic pricing.

          Background

          EZ Cap Reagent AG (3' OMe) is a co-transcriptional capping reagent for in vitro transcription of 5’ capped mRNA resulting in a Cap 1 structure. The Cap reagent has shown to provide up to 98% capping efficiency in literature.
          EZ Cap AG (3' OMe) contains the 5' N7-Methyl-3'-O-Methylguanosine similarly to mRNA capped using ARCA, which is different from EZ Cap AG with a 5' N7-Methylguanosine structure.
          EZ Cap AG requires an AG initiator. EZ Cap AG could typically provide >90% capped material with a naturally occurring Cap 1 structure. EZ Cap is compatible with wild-type T7 polymerase. 5 mM EZ Cap and rNTPs is recommended. Template design is an integral part of any transcription. EZ Cap reagent AG 3’OMe should be used with a specific initiating sequence 5’ AG 3’.
          Final capping efficiency is dependent on the EZ Cap Reagent, DNA template and final mRNA sequence. Secondary structure due to RNA length and base composition could affect final capping efficiency.

      • Properties
        • Categories
          Used for co-transcriptional capping of mRNA to form Cap 1 structure, m7(3'OMeG)(5')ppp(5')(2'OMeA)pG
          Alternative Name
          EZ Cap™ Reagent AG (3' OMe) for co-transcriptional capping of mRNA, m7(3'OMeG)(5')ppp(5')(2'OMeA)pG
          Molecular Formula
          C33H45N15O24P4 (free acid)
          Molecular Weight
          1159.6 (free acid)
          Appearance
          Solution
          Purity
          Purity ≥95% by AX-HPLC
          Storage
          Store at -20°C or below
          SMILES
          O=C1NC(N)=NC2=C1N=CN2C3C(O)C(O)C(COP([O-])(OC4C(OC)C(N5C(N=CN=C6N)=C6N=C5)OC4COP([O-])(OP([O-])(OP([O-])(OCC7OC(N8C=[N+](C)C9=C8N=C(N)NC9=O)C(O)C7OC)=O)=O)=O)=O)O3.[Na+].[Na+].[Na+]

          * For Research Use Only

      • Reference

        • 1. Denzil Furtado, Christina Cortez-Jugo, et al. "mRNA Treatment Rescues Niemann-Pick Disease Type C1 in Patient Fibroblasts." Mol Pharm. PMID: 36125338

    Documents

    COA (Certificate Of Analysis)

    MSDS (Material Safety Data Sheet)

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