EV Decorating Kit (For SA-Biotin)

This reagent is an efficient and versatile tool for protein modification. The transpeptidase sortase A(SA) can specifically recognize the conserved amino acid sequence LPXTG contained at the C-terminus of the protein, causing the peptide bond between threonine and glycine in LPXTG to break. At the same time, it forms an acylase intermediate with the polyglycine (GGG) at the N-terminus of the protein to form a new peptide bond, completing the peptide transfer reaction and achieving covalent binding between the two proteins. There is a GGG structure at the N-terminus of some proteins on the surface of exosomes, and synthetic peptides or recombinant target proteins result in LPXTG sequences at the C-terminus of the peptides/target proteins. Therefore, under the action of SA enzyme, the peptide/target protein will be covalently modified on the surface of extracellular vesicles.

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