EasyProbes™ Cell Viability Imaging Kit (Blue/Green)

EasyProbes™ Cell Viability Imaging Kit (Blue/Green) is a ready-to-use assay that can quickly and easily determine the viability of cells. Just add 2 drops each of EasyProbes™ Hoechst 33342 Live Cell Stain reagent and EasyProbes™ Green 488 Dead Cell Stain reagent to 1 mL of cell growth media, then determine viability by counting total vs dead cells. EasyProbes™ Hoechst 33342 reagent stains the nuclei of all the cells and can be detected with a standard DAPI filter. EasyProbes™ Green 488 Dead reagent stains only the nuclei of cells with compromised plasma membrane integrity and is detected using a standard FITC (green) filter set. This kit is appropriate for fluorescence microscopy and flow cytometry.

• EasyProbes™ Hoechst 33342 reagent: stains the nuclei of all cells; detected with a standard DAPI filter (excitation/emission maxima: 350/461 nm)
• EasyProbes™ Green 488 Dead reagent: stains only the nuclei of dead cells with compromised plasma membranes; detected with standard FITC (green) filter set (excitation/emission maxima: 500/530 nm)

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