CellCycle™ Green Stain

CellCycle™ Green Stain

Catalog Number:
CFA1277368ABP
Mfr. No.:
ABPB_A054
Price:
$220
  • Size:
    100 assays
    Quantity:
    Add to Cart:
      • Overview
        • Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a variety of physical, chemical, or biological means, to monitor apoptosis, and to study tumor behavior and suppressor gene mechanisms. In a given population, cells are distributed among three major phases of cell cycle: G0/G1 phase (one st of paired chromosomes per cell), S phase (DNA synthesis with variable amount of DNA), and G2/M phase (two sets of paired chromosomes per cell, prior to cell division). DNA content can be measured using fluorescent, DNA-selective stains that exhibit emission signals proportional to DNA mass. Flow cytometric analysis of these stained populations is then used to produce a frequency histogram that reveals the various cell cycle phases. This analysis is typically performed on permeabilized or fixed cells using a cell-impermeant nucleic acid stain, but is also possible using live cells and a cell-permeant nucleic acid stain.

          The CellCycle™ Green stain is DNA-selective, cell membrane-permeant, and nonfluorescent stain for DNA content analysis in living cells. The CellCycle™ Green stain is fluorescent upon binding to double-stranded DNA. CellCycle™ Green stain is excited using 488 nm laser with emission ~530 nm.

          The staining protocol is simple and includes incubating suspended cells in the presence of CellCycle™ stain and directly measuring the fluorescence without the need for any additional treatment or centrifugation steps. This live cell stain allows the simultaneous co-staining of the cell population for other parameters, and allows for the possibility of cell sorting based on DNA content.

          Please contact us at for specific academic pricing.

          More Details

      • Properties
        • Categories
          Cell Cycle Analysis
          Other Properties
          Excitation/Emission (nm): 500/530
          Storage
          4 °C, protect from light

          * For research use only

      • Applications
        • Application
          Flow cytometry

    Documents

    Protocol-A054

    Note: If you don't receive our verification email, do the following:

  • Copyright © Amerigo Scientific. All rights reserved.