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E. coli DNA ligase (60 U/μL)

E. coli DNA ligase (60 U/μL)

Catalog Number:

E001505821YEA

Mfr. No.:

14955ES82

Price:

$144

Size:

1200 U

Quantity:

Add to Cart:

- Overview
  - E. coli DNA ligase can catalyze the formation of phosphodiester bonds between the 5'-PO4 ends and 3'-OH ends of adjacent DNA chains, and has almost no obvious ligation activity for blunt-ended substrate DNA. The optimal pH for the reaction is 7.5-8.0 (Tris-HCl buffer), and the reaction requires NAD as a coenzyme. Compared with T4 DNA Ligase, which can connect sticky ends and blunt ends, this enzyme can only catalyze the connection between protruding end DNA, but if PEG and high concentrations of monovalent cations are present, it can also connect smooth-ended DNA. In addition, this product cannot catalyze the ligation reaction between DNA and RNA or between RNA.
    
    Components: E. coli DNA ligase (60 U/μL) (20 μL); 10×E. coli DNA ligase Buffer (100 μL); 10×BSA (0.05%) (100 μL)
    
    Please contact us at for specific academic pricing.
- Properties
  - Storage
    
    Store at -25~-15°C, valid for 1 year.
    
    Note
    
    It is normal if a small amount of precipitation appears when the buffer is dissolved. Please invert and mix before use.
    Please operate with lab coats and disposable gloves, for your safety.
    
    * For Research Use Only
- Applications
  - Application Description
    
    This enzyme can be used for ligation reactions in NGS methylation library construction, and can also be used for cDNA cloning using the Okayama-Berg method.

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