Endonuclease VIII (10 U/μL)

Endonuclease VIII is a DNA damage repair enzyme with N-glycosylase activity and AP-lyase activity. N-glycosylase activity can release damaged pyrimidine bases on double-stranded DNA to produce an apyrimidine (AP) site. AP-lyase activity cuts the phosphodiester bond at the AP site to produce gaps at 5' phosphate and 3' phosphate. The damaged bases recognized and removed by endonuclease VIII include: urea, 5,6-dihydroxythymine, thymine glycol, 5-hydroxy-5-methyldantoin, uracil glycol, 6-hydroxy-5,6-dihydrothymine and methylhydroxymalonyl urea.

Enzyme activity: 10 U/μL
Inactivation conditions: Incubate at 75°C for 10 min

1) Reaction system:
10 ×Uracil-Specific Excision Reagent Reaction Buffer: 2μL
dsDNA: X (10 pmol)
Uracil-Specific Excision Reagent (1 U/μL): 1μL
ddH2O: To 20μL

2) Reaction conditions: Mix thoroughly and react at 37°C for 30~60 min.

Components: Endonuclease VIII (100 μL/500 μL); 10 × Endonuclease VIII Reaction Buffer (1.5 mL/1.5 mL)

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