Name: Terminal Deoxynucleotidyl Transferase TdT
Price: 247.00 USD

- Overview
  - Terminal Deoxynucleotidyl Transferase (TdT) is a template-independent DNA polymerase that catalyzes the repeated addition of deoxynucleotides to the 3'hydroxyl end of oligonucleotides, single-stranded and double-stranded DNA. TdT reactions require a short sequence containing at least 3 bases as a primer. When using RNA as a template, TdT performance strictly depends on the tertiary structure of the 3'-end of the receptor RNA and the type of nucleotides. Generally, TdT is less efficient on RNA templates than on DNA templates.
    
    Enzyme activity definition: 1 activity unit (U) is defined as the amount of enzyme required to incorporate 1 nmol of deoxyribonucleotide into a polynucleotide fragment within 60 min at 37 °C.
    Inhibitors: Metal chelators, ammonium, chloride, iodide and phosphate ions.
    Inactivation conditions: Add EDTA and heat at 70°C for 10 min.
    
    Components: Terminal Deoxynucleotidyl Transferase (20 U/µL) (25 μL/125 μL); 5×Reaction Buffer (400 μL/2×1 mL)
    
    Please contact us at for specific academic pricing.
- Properties
  - Source
    
    Recombinant expression in E. coli
    
    Storage
    
    Store at -25~-15°C, valid for 1 year.
    
    Note
    
    5× Reaction Buffer contains CoCl2, so it is not compatible with downstream applications. The reaction mixture needs to be purified by column centrifugation or phenol/chloroform extraction and ethanol precipitation to remove CoCl2.
    Please operate with lab coats and disposable gloves, for your safety.
    
    * For Research Use Only

Terminal Deoxynucleotidyl Transferase TdT

Terminal Deoxynucleotidyl Transferase TdT

We Also Recommend

Endonuclease VIII (10 U/μL)

FuniCut® BamHI Restriction Endonuclease (BamHI Restriction Endonuclease Cutting Site G/GATCC)

Recombinant APOBEC3A (A3A) Protein Cytosine Deaminase