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Overview
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The dNTP solution provided by BioChain is specially manufactured and tested for downstream applications in molecular biology. The ready-to-use mix contains varied concentrations depending on the purpose. Some of the essential uses for this dNTP mix include PCR/RT-PCR, DNA labeling, and DNA sequencing. Our dNTP mix is also purified with preparative high-performance liquid chromatography (HPLC), which guarantees at least a 99.5% level of purity.
The product has undergone further rigorous quality testing:
• The concentration is verified by optical density spectrophotometry.
• Preparation is free of DNase contamination (determined by incubation of 4 dNTPs with radioactive substrates)
• Preparation is free of RNase contamination (determined by incubation of 4 dNTPs with radioactive substrates)
• Each lot of the product is tested for performance with Taq DNA polymerase.
Some of the important molecular biology applications using dNTP mixes include:
Polymerase Chain Reaction (PCR)
• Gene amplification for cloning
• RT-PCR for gene quantitation
DNA labeling
• Nick translational labeling using random nicks that are to be replaced using DNA polymerase
• PCR labeling to generate labeled DNA probes
• Oligonucleotide tailing to generate high sensitivity probes for hybridization studies
DNA sequencing
• Analyze a chain of DNA and determine the order of nucleotides within it
• Use for Sanger sequencing and more recent Next Gen Sequencing applicationsPlease contact us at for specific academic pricing.
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Overview