DAPI (hydrochloride)

- Overview
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    Background
    
    DAPI (hydrochloride) is a fluorescent probe which serves as a DNA-specific probe for flow cytometry, chromosome staining, as well as DNA visualization and quantitation in histochemistry and biochemistry. DAPI forms a fluorescent complex by attaching to the minor grove of consecutive (3 ~ 4 base pairs) A-T rich sequences of DNA. Although DAPI is capable of binding to other sequences of double stranded DNA, or double stranded RNA, these complexes are much less fluorescent. DAPI can be used alone for cell cycle studies of drug effects, or in combination with another fluorochrome for multi-parameter cell analysis. One of the most popular stains combines DAPI with sulforhodamine (SR 101) for simultaneous measurement of DNA and protein content in cells. DAPI can be used on either fixed or live cells, but its low permeability in live cells suggests that higher concentrations should be used.
    
    1. Kapuscinski J. DAPI: a DNA-specific fluorescent probe. Biotechnic & Histochemistry, 1995, 70(5): 220-233.
    2. Zink D, Sadoni N, Stelzer E. Visualizing chromatin and chromosomes in living cells. Methods, 2003, 29(1): 42-50.
- Properties
  - Categories
    
    A fluorescent probe, commonly used to stain DNA and chromosomes
    
    Alternative Name
    
    2-(4-carbamimidoylphenyl)-1H-indole-6-carboximidamide dihydrochloride
    
    CAS Number
    
    28718-90-3
    
    Molecular Formula
    
    C16H17Cl2N5
    
    Molecular Weight
    
    350.25
    
    Purity
    
    98.00%
    
    Solubility
    
    insoluble in EtOH; ≥10 mg/mL in H2O; ≥53.3 mg/mL in DMSO
    
    Storage
    
    Store at -20°C
    
    SMILES
    
    NC(C(C=C1)=CC=C1C2=CC3=CC=C(C(N)=N)C=C3N2)=N.Cl.Cl
    
    * For Research Use Only
- Reference
  - 1. Liang Y, Wang J. "Sorafenib-Induced Exosome Secretion Promotes Che-motherapy Resistance in Hepatoma Cell Line." Japanese J Gstro Hepato. 2021; V6(12): 1-7.

Documents

COA (Certificate Of Analysis)

MSDS (Material Safety Data Sheet)

Datasheet

DAPI (hydrochloride)

Background

Documents

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