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Overview
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This kit has been optimized for an efficient and fast purification of total DNA from :
Fresh/frozen stool samples.
Various food samples (raw material and processed food).
After the samples have been homogenized, the DNA can be extracted with the extraction buffer, lysis mixtures should be cleared by centrifugation or filtration in order to remove contaminants and residual cellular debris. The clear supernatant is then mixed with the binding buffer, proteinase K and isopropanol to create conditions for optimal binding to the silica membrane column. After washing with two different buffers for efficient removal of potential PCR inhibitors, DNA can be eluted in low salt buffer or water, and is ready-to-use in subsequent reactions.
Specifications:
• Silica Membrane Technology using MiniSpin columns.
• Sample size: up to 200mg.
• Complete removal of contaminants and inhibitors for reliable downstream applications.
• Simultaneous extraction of microbial DNA and host DNA.Please contact us at for specific academic pricing.
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Overview