DANAGENE SPIN FOOD-STOOL "Bacteria" Kit -for PCR-ready bacterial DNA extraction

This kit has been optimized for an efficient and fast PCR-ready bacterial DNA extraction (Listeria, Salmonella, E.coli, etc) from pre-enrichment or enrichment culture from different food samples, raw materials or feces using glass fiber membrane MicroSpin columns which selectively bounds the DNA.

Detection of very low levels of bacterial contamination in foods and feces necessitates these samples to be cultured for a few hours in an appropriate enrichment broth. The use of culture enrichment prior to PCR analysis serves many purposes, including:

1) Dilution of PCR­ inhibitory substances present in the sample matrix.
2) Multiplication of the target organism to provide detectable concentrations.
3) Dilution of dead cells.
4) Possibility of isolating the target organism for complementary tests.

The procedure includes a centrifugation of 1 ml of enrichment medium to concentrate the cells, the cells are lysed during an incubation period with Lysis Solution and Lysozyme (supplied with the kit). Then a digestion with Proteinase K (supplied with the kit) and cleaning of the lysis mixture by centrifugation and DNA binding to the glass fiber membranes packed in MicroSpin columns. The DNA that is bound is purified by several washes to eliminate the inhibitory potentials of the PCR and finally the DNA is eluted.

Specifications:
• Silica Membrane Technology using MiniSpin columns.
• Complete removal of PCR inhibitors.
• PCR and Real Time PCR- ready DNA.
• Sample size: from 1 ml of pre-enrichment or enrichment medium of different food samples.

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