Name: CyFlow™ CD4 PE
Price: 488.00 USD

- Overview
  - Please contact us at for specific academic pricing.
    
    Background
    
    CD4 (T4, Leu3a) is a 55 kDa single-chain transmembrane glycoprotein expressed on a subset of T lymphocytes (''helper'' T-cells) and also on monocytes, tissue macrophages and granulocytes. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1, 2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase.
- Properties
  - Host
    
    mouse
    
    Antibody Type
    
    monoclonal
    
    Isotype
    
    IgG1
    
    Reactivity
    
    human
    
    Clone
    
    MEM-241
    
    Immunogen
    
    2 N-terminal domains of human CD4 fused to human IgG1 Fc
    
    Conjugate
    
    PE
    
    Formulation
    
    The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
    
    Storage
    
    Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Leu3a, T4
    
    * Research use only
- Applications
  - Application
    
    Flow Cytometry
    
    Application Description
    
    The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
    
    Emission Maximum: 576 nm
    Excitation Maximum: 496 nm, 565 nm