Name: CyFlow™ CD4 PE
Price: 488.00 USD

- Overview
  - Please contact us at for specific academic pricing.
    
    Background
    
    CD4 (T4, Leu3a) is a 55 kDa single-chain transmembrane glycoprotein expressed on a subset of T lymphocytes (''helper'' T-cells) and also on monocytes, tissue macrophages and granulocytes. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1, 2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase.
- Properties
  - Host
    
    rat
    
    Antibody Type
    
    monoclonal
    
    Isotype
    
    IgG2b
    
    Reactivity
    
    mouse
    
    Clone
    
    GK1.5
    
    Immunogen
    
    Mouse CTL clone V4 cells
    
    Conjugate
    
    PE
    
    Formulation
    
    The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
    
    Storage
    
    Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
    
    Concentration
    
    0.5 mg/mL
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Leu3a, T4
    
    * Research use only
- Applications
  - Application
    
    Flow Cytometry
    
    Application Description
    
    The reagent is designed for Flow Cytometry analysis. Suggested working usage is 3·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
    
    Emission Maximum: 576 nm
    Excitation Maximum: 496 nm, 565 nm