Cy5 maleimide (non-sulfonated)

- Overview
  - Please contact us at for specific academic pricing.
    
    Background
    
    Cy5 maleimide is a mono-reactive dye for selectively labeling peptides and proteins which couples with thiol groups. The labeling reagent is a common fluorophore dye with low aqueous solubility which is compatible with various instrumentation like microscopes, imagers, and fluorescence readers. Cy5 maleimide is part of the probe among the biological research. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. The user can generate probes with virtually any non-genetically encoded moiety of interest with the scaffold in hand.
    G3C(Cy5) probe consists of two parts. First, a GGGC scaffold peptide is synthesized on solid phase, followed by coupling of the liberated peptide to Cy5 maleimide, while in solution [1].
    
    Reference: [1]. Popp, M.W. Site-Specific Labeling of Proteins via Sortase: Protocols for the Molecular Biologist. Methods in Molecular Biology,2015, 1266, 185–198.
- Properties
  - Categories
    
    Labeling of cysteine residues, as well as other thiolated molecules
    
    Alternative Name
    
    6-[(2E)-3,3-dimethyl-2-[(2E,4E)-5-(1,3,3-trimethylindol-1-ium-2-yl)penta-2,4-dienylidene]indol-1-yl]-N-[2-(2,5-dioxopyrrol-1-yl)ethyl]hexanamide
    
    Molecular Formula
    
    C38H45ClN4O3
    
    Molecular Weight
    
    641.24
    
    Appearance
    
    A solid
    
    Purity
    
    98.76%
    
    Solubility
    
    ≥64.1 mg/mL in DMSO; insoluble in H2O; ≥65 mg/mL in EtOH
    
    Storage
    
    24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
    
    SMILES
    
    CC1(C2=CC=CC=C2[N+](=C1C=CC=CC=C3C(C4=CC=CC=C4N3CCCCCC(=O)NCCN5C(=O)C=CC5=O)(C)C)C)C
    
    * For Research Use Only
- Reference
  - 1. Xueting Tang, Lin Chen, et al. "Lipophilic NO-Driven Nanomotors as Drug Balloon Coating for the Treatment of Atherosclerosis." Small. 2022 Aug 12;e2203238. PMID:35961946
    2. Qi Wang , Ting Li, et al. "Engineered Exosomes with Independent Module/Cascading Function for Therapy of Parkinson's Disease by Multistep Targeting and Multistage Intervention Method." Adv Mater. 2022 Apr 18;e2201406. PMID:35435282
    3. Xiuhua Pan, Xiaochen Pei, et al. "One-in-one individual package and delivery of CRISPR/Cas9 ribonucleoprotein using apoferritin." J Control Release. 2021 Sep 10;337:686-697. PMID:34389365
    4. Bhatt R, Chudaev M, et al. "Engineered EF-Tu and tRNA-Based FRET Screening Assay to Find Inhibitors of Protein Synthesis in Bacteria." Assay Drug Dev Technol. 2018 May/Jun;16(4):212-221. PMID:29870274