Name: Cy3-dUTP
Price: 276.00 USD

- Overview
  - Please contact us at for specific academic pricing.
    
    Background
    
    Cy3-dUTP can be used for direct enzymatic labeling of DNA/cDNA by PCR with Taq polymerase, Nick Translation with DNAse I/ DNA Polymerase I, cDNA labeling and 3’-end labeling. Cy3-dUTP can replace TTP in reactions in which it serves as a substrate for T4 and Taq DNA polymerases, E. coli DNA polymerase (holoenzyme and Klenow fragment), reverse transcriptase (from AMV and M-MuLV) and terminal transferase. The synthesized dye-labeled DNA/cDNA probes can be used for the identification of specific sequences by in-situ hybridization, microarray or blotting techniques. Cy3-dUTP can also be used for multicolor fluorescence labeling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Cy3-dUTP/dTTP ratio for PCR and Nick Translation: 30-50% Cy3-dUTP/50% dTTP.
- Properties
  - Categories
    
    Direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation
    
    Alternative Name
    
    Aminoallyl-dUTP - Cy3; 5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, labeled with Cy3, Triethylammonium salt, Cy3 dUTP, Cyanine 3-dUTP; 1-ethyl-2-((1E,3E)-3-(1-(6-(((E)-3-(1-((2R,4S,5R)-4-hydroxy-5-(((hydroxy((hydroxy(phosphonooxy)phosphoryl)oxy)phosphoryl)oxy)methyl)tetrahydrofuran-2-yl)-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)allyl)amino)-6-oxohexyl)-3,3-dimethyl-5-sulfoindolin-2-yli
    
    Molecular Formula
    
    C43H56N5O21P3S2(free acid)
    
    Molecular Weight
    
    1135.97 (free acid)
    
    Appearance
    
    Solution
    
    Purity
    
    95.00%
    
    Solubility
    
    Soluble in H2O
    
    Storage
    
    Store at -20°C or below
    
    SMILES
    
    O=S(C1=CC=C([N+](CC)=C(C2(C)C)/C=C/C=C(C(C)(C)C3=C4C=CC(S(O)(=O)=O)=C3)/N4CCCCCC(NC/C=C/C5=CN([[email protected]]6C[[email protected]](O)[[email protected]@H](COP(OP(O)(OP(O)(O)=O)=O)(O)=O)O6)C(NC5=O)=O)=O)C2=C1)([O-])=O
    
    * For Research Use Only

Documents

COA (Certificate Of Analysis)

MSDS (Material Safety Data Sheet)

Datasheet

Cy3-dUTP

Cy3-dUTP

Background

Documents

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