CPRG Kit -for Transfection Assay

CPRG assay kit is ideal to quantify low expression level of beta-Galactosidase. LacZ is one of the most frequently reporter gene used in transfection experiments. The LacZ encoded protein (beta-galactosidase, b-Gal), is very stable, resistant to proteolytic degradation and easily assayed.
The levels of active beta-Gal actosidase expression can be quickly measured by its catalytic hydrolysis of CPRG (Chlorophenol red-b-D-galactopyranoside), substrate to a dark red product. The high sensitivity of this substrate improves the measurement of beta-Galactosidase activity when the reporter gene expression is low. (Absorbance at 570-595 nm).
All the necessary reagents are provided in this assay kit and offer a rapid, simple and sensitive method to quantify spectrophotometrically the enzyme expression level in LacZ transfected cells.
Convenient for all transfection assays
Suitable for cultured cells and tissues
Simple, rapid and easy-to-use
Economical
Optimized with stable buffers
Kit contains: Lysis Buffer 55 mL, Standard Dillution Buffer 55 mL, 10X CPRG 5 mL, Substrate Buffer 55 mL, Stop Buffer 55 mL, Beta Gal Enzyme 100 µL

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