ONPG Kit -for Transfection Assay

ONPG assay kit is ideal to quantify high expression level of beta-Gal. The levels of active beta-Gal expression can be quickly measured by its catalytic hydrolysis of ONPG (o-nitrophenyl-beta-D-galactopyranoside) substrate to a bright yellow product (Absorbance at 405-420nm).LacZ is one of the most frequently reporter gene used in transfection experiments. The LacZ encoded protein (beta-galactosidase, beta-Gal), is very stable, resistant to proteolytic degradation and easily assayed.
All the necessary reagents are provided in this assay kit and offer a rapid, simple and sensitive method to quantify spectrophotometrically the enzyme expression level in LacZ transfected cells.
Convenient for all transfection assays
Suitable for cultured cells and tissues
Simple, rapid and easy-to-use
Economical
Optimized with stable buffers
Kit contains 5X Lysis Buffer 55 mL, Standard Dilution Buffer 55 mL, ONPG Substrate Solution 55 mL, Stop Buffer 55 mL, Beta Gal Enzyme Standard 100 µL

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