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Overview
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Comet Assay Kit enables DNA damage analysis based on comet tail length and fluorescence intensity. DNA molecules may experience strand breaks due to endogenous or exogenous DNA-damaging factors. Single nuclei are embedded in agarose gel and subjected to an electric field, causing DNA fragments to migrate towards the anode. The migration distance is related to the relative molecular mass and charge of the DNA fragments. After staining with a fluorescent dye, the results can be observed under a fluorescence microscope. If the cells are damaged, comet-like images will be visible.
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