Clotrimazole

- Overview
  - Clotrimazole is a broad-spectrum antifungal belonging to the imidazole subclass of azole compounds. These compounds interfere with the biosynthesis of ergosterol, a major membrane component of the fungal cytoplasmic membrane. Clotrimazole was discovered in 1969 and was developed by Schering Plough. It inhibits Ca2+-activated potassium channels and has promising anti-cancer effects. Clotrimazole is also a reversible inhibitor of several cytochrome P450 (CYP450) isoforms. In vitro-based CYP450 enzyme inhibition screening has been used to evaluate compound interactions.
    This product is considered a dangerous good. Quantities above 1 g may be subject to additional shipping fees. Please contact us for details.
    
    Please contact us at for specific academic pricing.
    
    Background
    
    Clotrimazole increases fungal cell permeability by inhibiting ergosterol synthesis, a major cell membrane component found exclusively in fungi, resulting in fungistatic properties. Specifically, it inhibits the microsomal cytochrome P450-dependent 14α-demethylase, which is critical to ergosterol biosynthesis.
- Properties
  - CAS Number
    
    23593-75-1
    
    Molecular Formula
    
    C22H17ClN2
    
    Molecular Weight
    
    344.84
    
    Appearance
    
    White crystalline powder
    
    Solubility
    
    Soluble in alcohol, acetone, DMSO, or chloroform. Practically insoluble in water.
    
    Other Properties
    
    Source: Synthetic
    Assay: (On Dried Basis): 98.5-100.5%
    Residue On Ignition: ≤0.1%
    Heavy Metals: ≤10ppm
    Loss on Drying: ≤0.5%
    Melting Point: 141- 145°C
    
    Storage
    
    2-8°C
    
    * For research use only
- Applications
  - Application Description
    
    Spectrum: Clotrimazole is broad-spectrum antifungal, targeting a broad range of fungi including Candida and Aspergillus species.
    
    Eukaryotic Cell Culture Applications: Clotrimazole can be used to inhibit CYP450 in cell cultures.
    
    Cancer Applications: Clotrimazole has promising anti-cancer properties, interfering with glycolytic enzymes, specifically their cellular distribution and activity. Clotrimazole induced a dose-dependent decrease in glucose uptake in three cell lines from human breast cancer cell lines (MCF10A, MCF-7 and MDA-MB-231), affecting the metabolism, growth, and migration. The compound was non-toxic to non-tumor human breast cell lines (Furtado et al, 2012).