ChomiX®-Magbeads Streptavidin (SA) Coated Magnetic Beads

Store at 4°C, avoid freeze-thaw during use.

· Before the first use, fully vortex and mix well to prevent from changing the magnetic bead concentration.
· Avoid prolonged ultrasound to prevent damage to the surface of beads. Prior to use, perform magnetic separation and washing 2-3 times with pure water or the buffer solution. Use beads as soon as possible to avoid long storage.

ChomiX®-Magbeads Streptavidin (SA) Coated Magnetic Beads are utilized in proteomics experiments for separation and enrichment of biotinylated proteins. First of all, their non-specific adsorption is compared with that of streptavidin agarose resin in 0.05% SDS / PBS buffer solution. For general proteome samples, both of them are washed with 0.05% SDS / PBS and high salt buffer solution after enrichment, followed by elution of the bound protein and silver staining analysis. As shown in Figure 1, ChomiX®-Magbeads Streptavidin (SA) Coated Magnetic Beads exhibit lower non-specific adsorption compared to the traditional streptavidin agarose. The proteome is labelled with biotin probe, enabling specifically covalent labeling of cysteine residues. After enrichment and identification via high-resolution mass spectrometry, the results display that the ratio of biotin probe-modified peptides to total peptides up to 97.4%, while the ratio of peptides in streptavidin agarose in the total is only 74.9% (Figure 2), indicating a strong biotin enrichment capacity and low non-specific adsorption of ChomiX®-Magbeads Streptavidin (SA) Coated Magnetic Beads.