Name: ChomiX®-PulldownKit–Streptavidin Magnetic Beads-Based Protein Enrichment Kit
Price: 799.00 USD

- Overview
  - This product enriches, separates and elutes proteins labeled with chemical probes (with alkynyl reporter groups) from the proteome for use in western blot experiments, using corresponding antibodies to detect target proteins.
    
    Please contact us at for specific academic pricing.
    
    Background
    
    There are various types of bioorthogonal coupling reactions, among which the most widely used are the Staudinger ligation, copper-catalyzed azide-alkyne cycloaddition (CuAAC), strain-promoted [3+2] cycloaddition (SPAAC), and tetrazine-involved Diels-Alder reaction (IEDDA).
    
    In the CuAAC reaction, a 1,4-disubstituted triazole linkage is formed, which is similar in size and geometry to an amide bond, exhibiting high thermal and chemical stability. This reaction can proceed rapidly in aqueous conditions, typically without producing byproducts, and is compatible with most functional groups. By controlling different ligands, reducing agents, and ratios, the alkyne-azide coupling reaction can be efficiently catalyzed in both homogeneous and heterogeneous reaction systems, and applied in protein fluorescent labeling, solid-phase carrier separation, among other areas. Copper-free reactions, including SPAAC and IEDDA, are particularly suitable for live cell systems, causing minimal damage to cells, hence finding application in live cell imaging, cell surface glycan labeling, and more.
- Properties
  - Other Properties
    
    Lysis buffer A: 10 mL * 1 tube
    Lysis buffer B: 10 mL * 1 tube
    Biotin-azide: 50X 0.25 mL * 1 tube
    BTTAA/Cu-Mix: 25X (Component A)0.5 mL * 1 tube
    Reducing agent: (Component B) 100 mg * 1 tube
    Reconstitution buffer: 10 mL * 2 tube
    Elution buffer: 0.7 mL * 1 tube
    Streptavidin Magbeads: 5 ml * 1 tube
    
    Storage
    
    Lysis buffer A: -20°C
    Lysis buffer B: -20°C
    Biotin-azide: -20°C
    BTTAA/Cu-Mix: -20°C
    Reducing agent: -20°C, protected from light
    Reconstitution buffer: -20°C
    Elution buffer: -20°C
    Streptavidin Magbeads: 4°C
    
    * For Research Use Only.
- Applications
  - Application Description
    
    It is suitable for enriching, separating, and eluting proteins labeled with chemical probes containing alkyne reporter groups from proteomes, enabling their use in western blot experiments with corresponding antibodies to detect target proteins.

ChomiX®-PulldownKit–Streptavidin Magnetic Beads-Based Protein Enrichment Kit

ChomiX®-PulldownKit–Streptavidin Magnetic Beads-Based Protein Enrichment Kit

Background

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