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Chemical Phosphorylation Reagent II

Chemical Phosphorylation Reagent II

Catalog Number:
MAN1137388MET
Mfr. No.:
103-10
Price:
$2,310
  • Size:
    10 g
    Quantity:
    Add to Cart:
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      • Overview

        • Chemical Phosphorylation Reagent (CPR II) contains a DMT group which can be left on the oligonucleotide and used for rapid purification of oligonucleotide 5'-phosphates by the popular DMTr-on technique, which employs disposable RP cartridges or “Trityl-on” RP HPLC purification. The DMTr group is removed with aqueous acid (e.g., 2%TFA in the case of Cartridge Purification) and the remaining linker is then eliminated after brief treatment with aqueous ammonium hydroxide (12 -15% ammonium hydroxide at room temperature for 15 minutes) to yield the 5'-phosphate.

          Please contact us at for specific academic pricing.

      • Properties
        • Categories
          Reagents for RNA and DNA synthesis
          Alternative Name
          [3-(4,4'-Dimethoxytrityloxy)-2,2-dicarboxyethyl]propyl-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite
          Appearance
          amorphous colorless glass
          Other Properties
          Coupling: 6 minute coupling time
          Storage
          1 year at -20°C (dry compound)

          * For research use only

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