cDNA – Human Adult Normal Tissue: Colon

cDNA – Human Adult Normal Tissue: Colon

Catalog Number:
C002096520BIO
Mfr. No.:
C1234090
Price:
$624
  • Size:
    40 rxn
    Quantity:
    Add to Cart:
      • Overview
        • Our PCR Ready First Strand cDNAs are available from different human adult normal tissues. 1 µl cDNA is sufficient for each PCR reaction.
          First-Strand cDNA is synthesized from RNA isolated from a wide variety of documented human adult and fetal normal tissues, human diseased and tumor tissues, mouse, rat, monkey and plant tissues. Total RNA used for cDNA synthesis is isolated by modified guanidine thiocyanate techniques. 10 µg total RNA was primed by an oligo dT primer and reverse transcription (RT) using MMLV reverse transcriptase in 40 µl final volume. RT reaction was stopped by heating at 65°C for 10 minutes. The cDNA is delivered in 1x RT buffer (1x RT Buffer: 50 mM Tris-Cl, pH 8.3, 75 mM KCl, 3 mM MgCl2, 10 mM DTT). 1 µl cDNA is sufficient for one PCR reaction. The 5′ end of human clathrin cDNA (a 6 kb gene) has been amplified by PCR from all of these cDNAs.

          Please contact us at for specific academic pricing.

      • Properties
        • Source
          Human
          Origin
          Colon
          Donor Information
          Male: 58 yrs old, 1 donor

          * Research Use Only

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