Cat tissue transglutaminase

- Overview
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    Zedira
- Properties
  - Name
    
    Tissue-type Transglutaminase, TG2, TGase 2, proteinglutamine-γ-glutamyltransferase
    
    Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    > 90% [by SDS-PAGE]
    
    Formulation
    
    The Transglutaminase is lyophilized from 50 mM Na-Phosphat pH 8, 150 mM NaCl. Sample contains maltodextrin.His6-fcTG2 is a Ca2+-dependent enzyme.
    
    Storage
    
    Store at -20 °C in working aliquots. Repeated freezing and thawing is not recommended. Delivery is possible at ambient temperature.
    
    Note
    
    INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.
    
    Molecular Weight
    
    78 kDa
    
    Class
    
    Animal Transglutaminase 2
    
    Reconstitution
    
    Add the volume of H2O the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots. Keep cooled on ice for short term storage.
    
    Activity
    
    Add 10 mM Ca2+ to activate His6-fcTG2.
- Applications
  - Application Description
    
    fcTG2 catalyzes acyl transfer reactions from glutamin residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. His6-fcTG2 may also be used for immunoprecipitation.

Datasheet

MSDS

Cat tissue transglutaminase

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