Cat 4MID® Kit - Format BB (2 tests)

- Overview
  - The Cat 4MID® Kit (Ref. 4VDX-19K10BB) contains all reagents and buffers required for the quantification of proAKAP4 protein in sperm of cats and all felines.
    
    This format BB of the Cat 4MID Kit (ref. 4VDX-19K10BB) allows from 12 to 24 analysis per kit.
    
    This ELISA Kit is the 1st functional test to evaluate sperm quality in cats (all species, all breeds and crossbred animals)
    
    The concentration of the proAKAP4 protein is a good indicator of sperm quality and of how the spermatozoa can keep its motility high and efficient overtimes (Sergeant et al. 2019 ; Delehedde et al. 2019).
    
    High concentrations of proAKAP4 help select cat semen with long lasting motility and are indicative of good spermatogenesis, giving more mature, more “normal’ spermatozoa and less tail abnormalities ratio in semen samples (Bastan and Akcay, 2021 ; Sergeant et al. 2019 ; Delehedde et al. 2019).
    
    Please contact us at for specific academic pricing.
    
    Background
    
    ProAKAP4 (A–kinase anchor protein 4) has been described over the last decade as a functional marker of spermatozoa. This key protein is largely conserved between species and its specific regulation during spermatogenesis seems essential to increase success rate in artificial insemination settings. Recent cross species analyses have identified the potential use of proAKAP4 as a pertinent biomarker of overall semen quality in clinical settings. In terms of structure, proAKAP4 is a precursor polypeptide that must be converted by motile and alive spermatozoa in mature AKAP4 that consequently will dock and coordinate the core transduction signals regulating sperm functionality, i.e motility, capacitating and fertility.
    
    1. Luconi M, Cantini G, Baldi E, et al. Role of A-kinase anchoring proteins (AKAPs) in reproduction. Front Biosci. 2011;16:1315–1330.
    2. Sergeant N, Briand-Amirat L, Bencharif D, et al. The sperm specific protein proAKAP4 as an innovative marker to evaluate sperm quality and fertility. Journal of Dairy and Veterinary Science. 2019;11:01‒19.
    3. Delehedde M, Blommaert D, Jouy N, et al. Concentration of proAKAP4 as a pertinent read-out of sperm quality in mammals. Animal Rep Sci. 2018;194:224.
    4. Delehedde M, Demouveaux B, Remy G, Selleslagh M et al. ProAKAP4 concentrations as an indicator of good spermatogenesis and sperm quality under oxidative stress conditions. Andrology. 2019;7(1):86.
    
    More Details
    
    4MID® ProAKAP4 ELISA Kits
- Properties
  - Details
    
    SPECIFICITY: Cat proAKAP4 (all breeds)
    ANALYTICAL RANGE: 0 ng to 150 ng
    SAMPLE TYPE: fresh, chilled and frozen semen / isolated spermatozoa
    
    * Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.
- Applications
  - Application
    
    ELISA
    
    Application Description
    
    The Cat 4MID® Kit allows to run 1 or 2 tests at the time. This innovative testing kit allows to quantify proAKAP4 in samples of cat semen and predicts sperm quality and male fertility over times.
    Composed of 12 breakable strips of 8 wells per plate/kit - allows from 12 to 24 analysis per kit
    This ELISA kit(96 wells microplate) allow to assess from 1 to 2 samples at the time - 12 breakable strips of 8 wells per plate/kit)Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.
- Reference
  - Carracedo S, Briand-Amirat L, Dordas-Perpinyà M, Ramos Escuredo Y, Delcombel R, Sergeant N and Delehedde M (2022) ProAKAP4 protein marker: towards a functional approach to male fertility. Animal Reproduction Science. Vol.247(107074):1-20. https://doi.org/10.1016/j.anireprosci.2022.107074
    Bastan I and Akcay E (2021) Quality assessment of frozen bull semen with the precursor A-kinase anchor protein 4 biomarker. Andrologia e14164.
    Riesco M, Anel-Lopez L, Neila-Montero M, Palacin-Martinez C, Montes-Garrido R, Alvarez M, de Paz P, Anel L (2020) ProAKAP4 as Novel Molecular Marker of Sperm Quality in Ram: An Integrative Study in Fresh, Cooled and Cryopreserved Sperm. Biomolecules. 10(7):1046. doi: 10.3390/biom10071046.
    Nixon B, Bernstein I, Cafe SL, Delehedde M, Sergeant S, Eamens AL, Lord T, Dun MD, De Iuliis GN and Bromfield EG (2019b) A Kinase Anchor Protein 4 is vulnerable to oxidative adduction in male germ cells. Frontiers in Cell and Developmental Biology – Vol. 7:319.
    Delehedde M, Carracedo S, Selleslagh M, et al. ProAKAP4 polypeptide as a biomarker of sperm functionality and male fertility disorders. Int J Gynecol and Reprod Sci. (2019);2(1):13-19.
    Sergeant N, Briand-Amirat L, Bencharif D and Delehedde M (2019) The Sperm Specific Protein ProAKAP4 as an innovative marker to evaluate sperm quality and fertility. Journal of Dairy & Veterinary Sciences. Vol. 11:01-19.
    Blommaert D, Sergeant N, Delehedde M, Jouy N, Mitchell V, Franck T, Donnay I, Lejeune JP and Serteyn D (2019) Expression, localization, and concentration of A-kinase anchor protein 4 (AKAP4) and its precursor (proAKAP4) in equine semen: Promising marker correlated to the total and progressive motility in thawed spermatozoa. Theriogenology. Vol. 131:52-60.
    Delehedde M, Blommaert D, Jouy N, Scabello J, Miersman H, Franck T, Serteyn D, Mitchell V and Sergeant S (2018) Concentration of proAKAP4 as a pertinent read-out of sperm quality in mammals. Animal Reproduction Science. Vol. 194:24.
    Sergeant N, Jumeau F, Eddarkaoui S, Sigala J, Dossou GF, Delehedde M, Buee L, Yvoz JF and Mitchell V (2016) Investigating proteomic methods and tools to assess sperm quality. Animal Reproduction Science. Vol. 169:99-135.
    Luconi M, Cantini G, Baldi E and Forti G (2011) Role of A-kinase anchoring proteins (AKAPs) in reproduction. Frontiers in Bioscience. Vol. 16:1315-1330.
    Miki K, Goulding EH, Fulcher KD, Eddy E, Willis WD and Brown PR (2002) Targeted disruption of the AKAP4 gene causes defects in sperm flagellum and motility. Developmental Biology. Vol. 248:331-342.

Cat 4MID® Kit - Format BB (2 tests)

Cat 4MID® Kit - Format BB (2 tests)

Background

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