Pig 4MID® Kit (proAKAP4 marker)

Innovative complete immunoassay to assess semen quality and fertility in boars.
The Pig 4MID® Kit (Ref. 4VDX-18K2) is the first immunoassay that validates objectively semen quality by quantifying a protein biomarker named proAKAP4 in samples of pig semen.

This ELISA kit is the 1st functional test to evaluate sperm quality in boars.

The dosage of the proAKAP4 biomarker with the 4MID® Kit, will bring you:

• Objective information on semen quality
• Boar Follow-ups
• Objective information of dose shelf-life (D=1, D+3, D+5)
• Traceability in the quality of the semen (reproducible results)
• A functional indicator of male fertility and prolificity (Sergeant et al. 2019)

The concentration of the proAKAP4 biomarker is a good indicator of sperm quality and of how the spermatozoa can keep its motility high and efficient overtimes.

Please contact us at for specific academic pricing.

Background

ProAKAP4 (A–kinase anchor protein 4) has been described over the last decade as a functional marker of spermatozoa. This key protein is largely conserved between species and its specific regulation during spermatogenesis seems essential to increase success rate in artificial insemination settings. Recent cross species analyses have identified the potential use of proAKAP4 as a pertinent biomarker of overall semen quality in clinical settings. In terms of structure, proAKAP4 is a precursor polypeptide that must be converted by motile and alive spermatozoa in mature AKAP4 that consequently will dock and coordinate the core transduction signals regulating sperm functionality, i.e motility, capacitating and fertility.

1. Luconi M, Cantini G, Baldi E, et al. Role of A-kinase anchoring proteins (AKAPs) in reproduction. Front Biosci. 2011;16:1315–1330.
2. Sergeant N, Briand-Amirat L, Bencharif D, et al. The sperm specific protein proAKAP4 as an innovative marker to evaluate sperm quality and fertility. Journal of Dairy and Veterinary Science. 2019;11:01‒19.
3. Delehedde M, Blommaert D, Jouy N, et al. Concentration of proAKAP4 as a pertinent read-out of sperm quality in mammals. Animal Rep Sci. 2018;194:224.
4. Delehedde M, Demouveaux B, Remy G, Selleslagh M et al. ProAKAP4 concentrations as an indicator of good spermatogenesis and sperm quality under oxidative stress conditions. Andrology. 2019;7(1):86.

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