Caspase-8 Colorimetric Assay Kit

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Background

Cysteine-dependent aspartate-directed proteases (Caspases) are a family of cysteine proteases that play important roles in apoptosis, necrosis, and inflammation. Sequential activation of caspases plays an important role in cell apoptosis. Caspase-8 is a caspase protein that cleaves and activates Caspase-3 and is involved in the programmed cell death induced by Fas and different apoptotic stimuli. Caspase-8 was detected in the insoluble fraction of the affected brain region in Huntington disease patients, which suggested it plays an important role in neurodegenerative diseases.
Caspase-8 Colorimetric Assay Kit provides a convenient and simple way for detecting the IETD-dependent caspase activity. When cleavage of IETD from the labeled substrate IETD-p-nitroaniline (IETD-pNA) by Caspase-8 or related caspases, the free pNA light emission can be quantified by using a microtiter plate reader or a spectrophotometer at 405 or 400 nm. Comparison of the absorbance of free pNA from an apoptotic sample with an uninduced control determines the fold increase in Caspase-8 activity.