Caspase-6 Colorimetric Assay Kit

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Background

Cysteine-dependent aspartate-directed proteases (Caspases) are a family of cysteine proteases that play important roles in apoptosis, necrosis, and inflammation. Sequential activation of caspases plays an important role in cell apoptosis. Caspase-6 is a member of the caspase family. Caspase-6 is processed by caspases 7, 8 and 10, and can also undergo self-processing. Caspase-6 acts as a downstream enzyme in the caspase activation cascade and interacts with Caspase 8.
Caspase-6 Colorimetric Assay Kit provides a convenient and simple way for detecting the VEID-dependent caspase activity. When cleavage of VEID from the labeled substrate VEID-p-nitroaniline (VEID-pNA) by Caspase-6 or related caspases, the free pNA light emission can be quantified by using a microtiter plate reader or a spectrophotometer at 405 or 400 nm. Comparison of the absorbance of free pNA from an apoptotic sample with an uninduced control determines the fold increase in Caspase-6 activity.