Carboxylated - 96 well Solid plates

Carboxylated Plates 96-Well Solid Plates: Solid format designed for high-performance assays.

Carboxylated Plates are ideal for promoting the covalent immobilization of compounds with reactive free amino groups. The surface features a negatively charged carboxylated layer, enabling the binding of peptides and other molecules through specific covalent bonds using EDC-mediated amination. This method overcomes several limitations, including the immobilization of small molecules (1,000–5,000 Da), such as peptides, drugs, toxins, or hormones, which may otherwise bind weakly or not at all through physical adsorption. Additionally, it ensures oriented immobilization, maintaining the integrity and accessibility of specific sites, and offers increased storage stability by minimizing the risk of spontaneous desorption.

Carboxyl Treated 96 Well Plates are available in clear, white, and black polystyrene in Breakable Strip, Strip and Solid format. They are suitable for ELISA, Luminescence, Fluorescence, and Chemiluminescent assays.

Surface:
The Carboxylated Surface is designed to facilitate the covalent immobilization of compounds containing reactive free amino groups through EDC-mediated amination. This immobilization method addresses limitations associated with physical adsorption, particularly for small molecules such as peptides (1,000–5,000 Da), drugs, toxins, and hormones. By promoting oriented immobilization, it ensures that the molecules retain both their integrity and accessibility of specific active sites, making these plates ideal for sensitive assays requiring precise molecular interactions.

Key features of Carboxylated Plates
· Enhanced binding efficiency for small molecules like peptides (1,000–5,000 Da), drugs, toxins, and hormones, which typically struggle with physical adsorption.
· Oriented immobilization, ensuring that molecules retain their structural integrity and maintain accessible active sites for optimal functionality.
· Improved storage stability, significantly reducing the risk of spontaneous desorption, leading to more reliable and long-lasting immobilization compared to physical adsorption methods.
· Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
· Wells design: flat bottom.
· Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
· Colors: Available in clear, white, and black. Customizable upper rim colors upon request.
Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
· Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
· Alphanumeric Coding: For easy and accurate well identification during experiments.
· Recommended working volume: of 75 to 200 μl
· Packaging: 5 plates in a transparent bag.
· Unit: Contains 5 plates
· Minimum order: 10 plates.
· Ready to use

Key Benefits
· Plates Design:
The mould design provides optical quality, important to reduce the background signal.
The rim protects the external face of the bottom from scratches.
· Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
· Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
· Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
· Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.

Please contact us at for specific academic pricing.

Background

Carboxylated Surface is dedicated to promote the covalent immobilization of compounds containing reactive free amino groups using the EDC mediated amination.
Surfaces with carboxylic groups covalently bound are dedicated to promote the covalent immobilization of compounds containing reactive free amino groups using the EDC mediated amination.

This kind of immobilization can overcome some of the limitations connected with physical adsorption of the molecules to the surfaces:

immobilization of molecules which are bound weakly or not all by physical adsorption, namely small peptides (M.W. 1,000 – 5,000 Da) drugs, toxin or hormones
oriented immobilization of molecules in order to secure the integrity and accessibility of their specific sites
increased storage stability compared with that of physical adsorption because of the reduced risk of spontaneous desorption
Coupling molecules having an amino group to COOH surface
The amino group presents in any molecules, such as peptides or proteins, binds to Biomat Carboxylated Surface through formation of amide bonds between the amino group presents in the molecule and the surface carboxylic group by the action of carbodiimide.

· Carboxylated Surfaces main features
Biomat Carboxylated products have the following characteristics:

Ready to Use
Manufactured under ISO:9001 guidelines
All lots are tested for uniformity and reproducibility
Certificate of Quality is released for every lot
For Research Use Only
Also, Carboxylated Products ensures:

Storage and Stability
The Carboxylated products are stored at room temperature in sealed PE bags and are stable until the expiration date printed on the label.

Carboxylated Microplates Specifications
Treatment
The surface is modified with carboxylic groups.
Volume of treatment 200 µl/well.

· Carboxylated PCR 8 Strip Tubes and PCR Plates Specifications
Treatment
The surface is modified with carboxylic groups.
Volume of treatment 200 µl/well.