Carbenicillin Disodium, CulturePure®

Carbenicillin Disodium, CulturePure® is a carboxypenicillin bacteriolytic antibiotic and is routinely used in microbiology and plant biology as a selection agent to isolate resistant E. coli and transformed plants, respectively.
The compound interferes with peptidoglycan synthesis, thus it can also be used to study the role of penicillin-sensitive transpeptidases in the formation of cell walls, selection of ampr transformed cells and to study the role of penicillin-sensitive transpeptidases in cell wall biosynthesis. The carboxycillins are susceptible to degradation by β-lactamase enzymes.

Carbenicillin Disodium, CulturePure is soluble in water.
CulturePure products are uniquely designed for cell/plant culture applications. They have been carefully tested to make sure they do not exhibit toxicity against routine cell lines or plant cells.

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Background

Carbenicillin disodium interferes with PBP (penicillin binding protein) activity involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues providing additional strength to the cell wall. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised and ultimately leads to cell lysis and death. Resistance to β-lactams is commonly due to cells containing plasmid encoded β-lactamases.