Calcein blue, AM

Calcein blue, AM

Catalog Number:
CSA1277388ABP
Mfr. No.:
ABPB_C022; ABPB_C023
Price:
$270
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      • Overview
        • Calcein Blue, AM is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity and cell-membrane integrity, which is required for intracellular retention of their fluorescent product. Calcein blue AM is only weakly fluorescent (excitation/emission maxima ~322/435 nm). Upon cleavage of the AM esters by intracellular esterases, this tracer becomes relatively polar and is retained by cells for several hours. In addition, its fluorescence intensity increases and its fluorescence spectra shift to longer wavelengths, with excitation/emission maxima of ~360/449 nm. This dye is provided as 1 mg solid (C022) and 1 mg/mL solution in DMSO (C023).

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          More Details

      • Properties
        • Categories
          Cyrosol stains
          Molecular Weight
          465.41
          Other Properties
          Excitation/Emission (nm): 360/449
          Storage
          -20°C, protect from light

          * For research use only

      • Applications
        • Application
          Cell labeling; cell tracker
      • Reference
        • Towards a Non-Terminal Viability Assay for Foraminiferan Protists. Bernhard JM, Newkirk SG, Bowser SS, J Euk Microbiol (1995) 42:357-357
          RANK ligand-induced elevation of cytosolic Ca2+ accelerates nuclear translocation of nuclear factor kappa B in osteoclasts. Komarova SV, Pilkington MF, Weidema AF, Dixon SJ, Sims SM, J Biol Chem (2003) 278:8286-8293
          Whole animal cell sorting of Drosophila embryos. Krasnow MA, Cumberledge S, Manning G, Herzenberg LA, Nolan GP, Science (1991) 251:81-85

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