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BCECF

BCECF

Catalog Number:
CSA1277390ABP
Mfr. No.:
ABPB_C027
Price:
$342
  • Size:
    5 mg
    Quantity:
    Add to Cart:
    Add to Cart
      • Overview

        • BCECF is an ideal dual-excitation ratiometric pH indicator for measuring changes ± 1 pH unit around pH 7 with a pKa of ~6.98. pH measurements with BCECF are made by determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at ~490 nm versus the emission intensity when excited at its isobestic point of ~440 nm.

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          More Details

          EasyProbe™ Cell Structure Probes
      • Properties
        • Categories
          Cyrosol stains
          Molecular Weight
          520.45
          Other Properties
          Excitation/Emission (nm): 490/535
          Storage
          2-8°C, protect from light

          * For research use only

      • Applications
        • Application
          Cell labeling; pH indicator
      • Reference

        • Fluorescent indicators for intracellular pH. Han J, Burgess K, Chem Rev (2010) 110:2709-2728
          Wavelength Considerations in Confocal Microscopy of Botanical Specimens. Fricker MD, White NS, J Microsc (1992) 166:29-29
          Calibration Methods and Avoidance of Errors in Measurement of Intracellular pH (pHcyt) Using the Indicator Bis (Carboxyethyl)-5(6)-Carboxyfluorescein (BCECF) in Human Platelets. Valant PA, Haynes DH, J Fluorescence (1992) 2:191-191

    MSDS

    Documents

    Protocol-C027+C028+C029

    COA-C027

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