C6 Maleimide Activated HRP

This is a replacement product for CM53211. C6 Maleimide activated HRP is much more stable than previously offered product CM53211.

The C6 maleimide activated horseradish peroxidase (HRP) is designed for direct conjugation of HRP with proteins, peptides, or other ligands containing free sulfhydryl groups for use as an enzyme-linked detection reagent. This product consists of HRP that has been modified with EMCS (N-Ɛ-maleimidocarproyl oxysuccinimide ester, 9.4 Å spacer length) to attach 1 to 2 maleimide groups per HRP molecule while retaining the peroxidase activity. The activated HRP will covalently attach to sulfhydryl-containing compounds to generate the majority single labeled HRP-conjugates reporter probes.

The product is sold as 1 vial of 2 mg (Cat# CM53214-1x2MG) or 3 vials of 2 mg (Cat# CM53214-3x2MG).

• Key Features of this Maleimide activated HRP
Lyophilized powder ready for conjugation after reconstitution with water, no need for external buffer.
Suitable for single labeling with an average maleimide groups per HRP of 1-2
High purity: ≥99% by SEC HPLC
High activity: >350 units/mg protein

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