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Coomassie-stained SDS-PAGE. Lane 1: Molecular weight markers; Lane 2: 5 µg purified Bovine immuno 20S proteasome

Coomassie-stained native-PAGE. Lane 1: 5 µg purified Bovine immuno 20S proteasome

Activation of 5 nM immuno 20S proteasome (Cat. # A1500) by 25 nM PA28α (Cat. # A2100), the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.

Activation of 5 nM immuno 20S proteasome (Cat. # A1500) by 25 nM PA28β (Cat. # A2200), the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.

Activation of 5 nM immuno 20S proteasome (Cat. # A1500) by 25 nM PA28γ (Cat. # A2300), the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.

Bovine immuno 20S proteasome

Bovine immuno 20S proteasome

Catalog Number:

PAS1097542UBP

Mfr. No.:

A1500

Price:

$405

Size:

25 µg

Quantity:

Add to Cart:

- Overview
  - Upon stimulation with IFN – γ, the expression of the three catalytic β subunits β1, β2, and β5 with iso-forms β1i (LMP2), β2i (MECL – 1), and β5i (LMP7) are induced, respectively. These subunits are incorporated into the 20S proteasome to form the immune 20S proteasome. It was reported that the immunoproteasome has altered proteolytic activities compared to its normal form, which favor the generation of immunopeptides for antigen presentation.
    
    Please contact us at for specific academic pricing.
- Properties
  - Protein Name
    
    Immuno 20S proteasome
    
    Source
    
    Bovine red blood cells
    
    Formulation
    
    20 mM Tris, 20 mM NaCl, 1 mM EDTA, 5 mM βME, 10% Glycerol
    
    Storage
    
    Store at -80°C; avoid multiple freeze-thaw cycles
    
    Concentration
    
    See tube label
    
    Molecular Weight
    
    700 kDa
    
    * Research Use Only

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