Login / Register

0

Coomassie-stained native-PAGE. Lane 1: 5 µg purified Bovine 20S proteasome

Coomassie-stained native-PAGE. Lane 1: 5 µg purified Bovine 20S proteasome

Activation of 5 nM 20S proteasome by 25 nM PA700 (Cat. # A1300), the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.

Activation of 5 nM 20S proteasome by 25 nM PA28α (Cat. # A2100), the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.

Activation of 5 nM 20S proteasome (Cat. # A1400) by 25 nM PA28β (Cat. # A2200), the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.

Bovine 20S proteasome

Bovine 20S proteasome

Catalog Number:

PAS1097546UBP

Mfr. No.:

A1400

Price:

$315

Size:

25 µg

Quantity:

Add to Cart:

- Overview
  - The 20S proteasome has a barrel – shaped structure arranged as four heptomeric rings of αββα. In eukaryotes, each of α and β ring is composed of seven different proteins. The β1, β2 and β5 subunits have ‘caspase-like’, ‘trypsin-like’ and ‘chymotypsin-like’ activities, respectively. In 26S proteasome-mediated protein degradation, to entry the β chamber of the 20S proteasome that houses the proteolytic sites, a substrate protein has to pass through a substrate translocation channel consisting of the double-ring formed by six ATPases of PA700 and the α chamber formed by α subunits of the 20S proteasome.
    
    Please contact us at for specific academic pricing.
- Properties
  - Protein Name
    
    20S proteasome
    
    Source
    
    Bovine red blood cells
    
    Formulation
    
    20 mM Tris, 20 mM NaCl, 1 mM EDTA, 5 mM βME, 10% Glycerol
    
    Storage
    
    Store at -80°C; avoid multiple freeze-thaw cycles
    
    Concentration
    
    See tube label
    
    Molecular Weight
    
    700 kDa
    
    * Research Use Only

We Also Recommend

Bovine 19S (PA700)

Bovine 19S (PA700)

$816

Bovine 19S (PA700)

Bovine 19S (PA700)

$480

Human 26S proteasome

Human 26S proteasome

$872

Human 26S proteasome

Human 26S proteasome

$520

Note: If you don't receive our verification email, do the following:

Confirm that you entered your email address correctly.
Check if the email is in your spam or junk folder.
Or you may contact us at .

Products

Amerigo Scientific

Support

Contact Us

Copyright © Amerigo Scientific. All rights reserved.