Blood DNA Midi Kit (1-5mL) Blood Isolation Midi Kit For DNA From Blood

Quickly purify high-quality genomic DNA from anticoagulated blood (1-5mL).
• No RNase contamination: The DNA-Only Column provided by the kit makes it possible to remove the RNA from the genomic DNA without adding RNase during the experiment, preventing the laboratory from being contaminated by exogenous RNase.
• Fast speed: Protease has higher activity than similar proteases, and digests tissue samples quickly; the operation is simple, and the genomic DNA extraction operation can be completed within 20-80 minutes.
• Convenient: The centrifugation is performed at room temperature, and there is no need for 4°C low-temperature centrifugation or ethanol precipitation of DNA.
• Safety: No organic reagent extraction is required.
• High quality: The extracted genomic DNA has large fragments, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.
• Micro-elution system: It can increase the concentration of genomic DNA, which is convenient for downstream detection or experiment.

Please contact us at for specific academic pricing.

Background

The blood sample contains a large number of red blood cells and platelet cells, which makes the blood processing more troublesome. However, the blood genomic DNA extracted by the general method is not high in purity and the content is too low. This kit uses the brand-new Protease Plus and unique BL1 and BL2 buffer systems, which can completely digest anticoagulated blood and coagulation samples in the shortest time, thereby avoiding DNA degradation to the greatest extent and obtaining the largest amount of genomic DNA. The fast blood processing system and simple spin column operation greatly simplify the extraction of blood genome, so that high-quality, high-purity genomic DNA can be obtained within 40 minutes.
The DNA-only silica gel membrane used in the spin column of this kit is a unique new material of the company, which efficiently and specifically adsorbs DNA, and can remove RNA, impurity proteins, ions and other organic compounds in cells to the greatest extent. The obtained DNA fragments are large, with high purity, stable and reliable quality, and the maximum carrying capacity of a spin column is 80μg DNA. The obtained DNA can be used for molecular biology experiments such as restriction digestion, PCR, Southern hybridization, library construction and so on. The kit can process up to 5mL of blood at a time to obtain a sufficient amount of high-purity genomic DNA.