Cell-Free DNA Isolation Kit

Isolation of cfDNA from plasma samples.
• Efficient Extraction-High DNA extraction yield, good purity,effective removal of impurities and downstream PCR inhibitors.
• Flexible Sample Incorporation-Easy extraction of cfDNA from 0.5mL to 4mL samples with elution volumes as low as 20uL.
• Diverse Applications-Validated by clinical samples, designed for tumor, infection and prenatal diagnosis with liquid biopsy.
• Wide support of Automated Platform-Compatible with manual ans automated extraction procedures, support Tecan, Hamilton, PE and other nucleic acid extraction workstations.

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Background

Cell-free DNA (cfDNA) is a fragment of cfDNA released from cells into the bloodstream that can carry biological information from tumors, pathogens and fetuses. The analysis of cfDNA provides key markers for diagnosis, detection and monitoring of disease progression. Currently, this technology has been widely used in the fields of tumor mutation detection, targeted drug guidance and prognosis,pathogen detection and non-invasive prenatal screening.
High quality nucleic acid is the guarantee for genomics research. We uses superparamagnetic bead purification technology to provide users with a highly sensitive nucleic acid extraction solution that easily achieves trace nucleic acid extraction for low concentration. As verified by thousands of clinical samples, this product can extract high quality cfDNA from cell-free body fluids such as fresh or frozen plasma, serum, pleural fluid, ascites and cerebrospinal fluid for scientific research and clinical in-vitro diagnostic use.
The Cell-free DNA Isolation Kit is designed for isolating circulating cell-free DNA (cfDNA) from human plasma, serum, body fluid, or urine samples. The kit can quickly and conveniently isolate cfDNA in three steps: lysis/binding, washing and elution. It uses specially designed silica-coated superparamagnetic beads with a unique lysis/binding system to recover circulating nucleic acids by hydrogen bonding and electrostatic forces, without adsorbing proteins and other impurities. It is suitable for high-throughput automated extraction work-stations in preparation for any downstream analysis using PCR or NGS.