BlasTaq™ Probe One-Step RT-qPCR

- Overview
  - BlasTaq™ Probe One-Step RT-qPCR provides convenience using the RT-qPCR Enzyme Mix and BlasTaq™ Probe 2X qPCR MasterMix for both highly sensitive and specific reverse transcription and TaqMan probe-based real-time qPCR amplification in a single reaction tube. Our proprietary RT-qPCR Enzyme Mix contains our next generation BlasTaq™ DNA Polymerase as well as stabilizers and enhancers that optimize the two reactions in a “single step”. This offers the end user flexibility with RNA templates and primer selection, an efficient and easy-to-use set up, and a reliable alternative to conventional “two-step” sequential RT-qPCR. The MasterMix is suitable for SNP genotyping assays, gene expression analysis, microarray validation, and high throughput screening applications. ROX reference dye is provided separately, making it universally compatible with most qPCR instruments.
    
    Product Features:
    Simple, one-step RT-qPCR reaction
    Flexibility with RNA templates and primer selection
    ROX reference dye provided separately
    Universally compatible with most qPCR instruments
    Earn 2X the rewards points
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    qPCR MasterMixes and Kits
    
    Storage
    
    Store at -20°C.
    
    * For research use only. Not for diagnostic or therapeutic use.
- Reference
  - Lee, G et al. "Potential monoclonal antibody therapy for the treatment of ovarian cancer" Ovarian cancer—basic science perspective :385-406 (2012). DOI: 10.5772/1268 . Application: Reverse Transcription.
    Wu, F., Niu, Z., Zhou, B., Li, P., & Qian, F. "PSMB1 Negatively Regulates the Innate Antiviral Immunity by Facilitating Degradation of IKK-ε" Viruses 11(2):99 (2019). DOI: 10.3390/v11020099.
    Zhao, G., Zhang, T., Sun, H. J., & Liu, J. "Copper nanoparticles induce zebrafish intestinal defects via endoplasmic reticulum and oxidative stress" Metallomics. : (2019).
    Yang, S., Tian, M., & Johnson, A. N. (2020). SARS-CoV-2 protein ORF3a is pathogenic inDrosophilaand causes phenotypes associated with COVID-19 post-viral syndrome. SARS-CoV-2 Protein ORF3a Is Pathogenic in Drosophila and Causes Phenotypes Associated with COVID-19 Post-Viral Syndrome. Published. https://doi.org/10.1101/2020.12.20.423533
    Yang, Y. W., Meng, X., Meng, Y. Y., Tang, H. K., Cheng, M. H., Zhang, Z. Q., Xu, W. Q., & Long, W. (2021). ceRNA regulatory network of FIH inhibitor as a radioprotector for gastrointestinal toxicity by activating the HIF-1 pathway. Molecular Therapy - Nucleic Acids, 25, 173–185. https://doi.org/10.1016/j.omtn.2021.05.008
    Luo, N., Cheng, W., Zhou, Y., Gu, B., Zhao, Z., & Zhao, Y. (2021). Screening Candidate Genes Regulating Placental Development from Trophoblast Transcriptome at Early Pregnancy in Dazu Black Goats (Capra hircus). Animals, 11(7), 2132. https://doi.org/10.3390/ani11072132

Datasheet

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BlasTaq™ Probe One-Step RT-qPCR

BlasTaq™ Probe One-Step RT-qPCR

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